Ultrasound-Powered Wireless Underwater Acoustic Identification Tags for Backscatter Communication.

Autonomous underwater vehicle (AUV) operations are limited by currently achievable underwater localization and navigation solutions; hence, the development of low-cost and passive (i.e., operable without an active power supply) acoustic underwater markers (or tags) can provide accurate localization information to AUVs improving their situational awareness, especially when operating in small scales or confined missions. This work presents an acoustic identification (AID) tag that can be powered wirelessly with ultrasonic power transfer from a remote acoustic source (e.g., mounted on an interrogating AUV) and provide localization information using backscatter communication. The AID tag harvests energy from the acoustic signal generated from the AUV and communicates by modulating the reflected signals from an embedded piezoelectric transducer. A scaled broadband AID tag prototype that achieves concurrent acoustic energy harvesting (tuned around 1.3 MHz) and backscatter communication (in wider frequency band 600 and 800 kHz) using frequency-domain multiplexing is implemented using a custom broadband impedance matching-based transducer design approach. During concurrent power and data operation, this prototype AID tag achieves data rates up to 200 kb/s using amplitude- and frequency-based modulation communication. The use of broadband schemes to achieve robust communications in low SNR (tested here down to -6 dB) is also demonstrated using linear frequency-modulated data carriers. Finally, the extension to full-scale devices of this AID tag concept and potential applications for short-range AUV routing and navigation such as homing and docking are discussed.

Surveilling brain damage using brain biomarkers in hypoglycemic neonatal calves with diarrhea.

Hypoglycemia is a condition associated with neonatal diarrhea in calves, leading to increased mortality and neurological clinical signs. The aim of the present study was to determine the development of brain damage in hypoglycemic calves with neonatal diarrhea and the diagnostic and prognostic significance of these biomarkers. Ten healthy and 50 hypoglycemic calves with diarrhea were included in the study. Clinical examination, blood gases and complete blood count were performed at admission. Blood serum calcium-binding protein B (S100B), neuron-specific enolase (NSE), glial fibrillary acidic protein (GFAP), ubiquitin carboxyl-terminal hydrolysis isoenzyme-1 (UCHL-1), activitin A (ACT), adrenomodullin (AM) concentrations, and creatine kinase-BB (CK-BB) enzyme activity were measured using commercial bovine-specific ELISA kits to assess brain damage. Of the hypoglycemic calves enrolled in the study, 13 (26%) survived and 37 (74%) died. In addition, 32 (64%) of the calves had severe acidosis and 24 (48%) had sepsis. S100B, GFAP, UCHL-1, CK-BB (p < 0.001) and NSE (p < 0.05) concentrations were significantly higher in hypoglycemic calves compared to healthy calves, while ACT concentrations were lower. Blood glucose concentration was negatively correlated with serum S100B, GFAP, UCHL-1, and CK-BB enzyme activity and positively correlated with ACT in hypoglycemic calves (p < 0.01). Brain injury biomarkers were not predictive of mortality (p > 0.05). Morever, severe hypoglycemia, severe acidosis and sepsis variables were not found to have sufficient capacity to predict mortality when considered alone or together (p > 0.05). In conclusion, brain damage may develop as a consequence of hypoglycemia in calves. S100B, NSE, GFAP, UCHL-1, ACT, and CK-BB concentrations can be used to diagnose brain damage in hypoglycemic calves. However, the variables of severe hypoglycemia, severe acidosis, and sepsis together with the biomarkers of brain injury have a limited value in predicting the prognosis of neonatal calves with diarrhea.

Investigation of gastrointestinal injury-related biomarkers in dairy cattle with displaced abomasum.

BACKGROUND: Displaced abomasum (DA) is one of the most important metabolic disorders of dairy cattle. In DA, ischaemic damage may occur as a result of impaired perfusion due to abomasal displacement, which may result in gastrointestinal mucosal damage. OBJECTIVE: Investigation of gastrointestinal tissue damage in cattle with right displacement of the abomasum (RDA) and left displacement of the abomasum (LDA) using intestinal-related biomarkers. METHODS: Forty-eight DA (24 LDA, 24 RDA) and 15 healthy Holstein dairy cows were enrolled between March 2021 and July 2022. Serum biomarkers including gamma-enteric smooth muscle actin (ACTG-2), liver-fatty acid binding proteins (L-FABP), platelet activating factor (PAF), trefoil factor-3 (TFF-3), leptin, claudin-3 and interleukin-8 (IL-8) concentrations were measured from venous blood samples. RESULTS: L-FABP concentrations in the LDA group and TFF-3 concentrations in the RDA group were lower than in the control group. The leptin concentration of the RDA group was higher than that of the other groups. There was a negative correlation between lactate, leptin and IL-8 concentrations. There was a negative correlation between lactate and TFF-3, whereas leptin and lactate were positively correlated. Leptin was the more reliable biomarker for discriminating between RDA and LDA cases. CONCLUSION: Changes in serum L-FABP, TFF-3 and leptin concentrations in cattle with DA may reflect acute intestinal injury and the subsequent repair phase. However, these biomarkers had poor diagnostic performance in discriminating between healthy and cattle with DA, while leptin emerged as the most useful marker in differentiating LDA from RDA cases.

Acute Kidney Injury Is Associated with Higher Serum Cys-C and NGAL Concentrations, and Risk of Mortality in Premature Calves with Respiratory Distress Syndrome.

The purpose of the present study was to establish the development of acute kidney injury (AKI) and evaluate the usefulness of kidney-specific biomarkers in diagnosing AKI in premature calves with respiratory distress syndrome (RDS). Ten-term healthy and 70 premature calves with RDS were enrolled. Clinical examination, blood gases, and chemical analysis were performed at admission and 72 h. Serum concentrations of blood urea nitrogen (BUN), creatinine (Cre), phosphorus (P), cystatin-C (Cys-C), neutrophil gelatinase-associated lipocalin (NGAL), uromodulin (UMOD), and liver-type fatty acid-binding protein (L-FABP) were measured to evaluate kidney injury. Our findings showed that 38.5% of the premature calves with RDS developed AKI. The RDS-AKI group had a 4-fold higher mortality risk than the RDS-non-AKI group. Cys-C, with 90% and 89% specificity, and NGAL, with 100% sensitivity and 85% specificity, were the most reliable biomarkers to determine AKI in premature calves. The usefulness of any biomarker to predict mortality was not found to be convincing. In conclusion, AKI can develop as a consequence of hypoxia in premature calves and may increase the risk of mortality. In addition, serum Cys-C and NGAL concentrations may be useful in the diagnosis of AKI in premature calves with RDS.

Optimizing transcranial ultrasound delivery at large incident angles by leveraging cranial leaky guided wave dispersion.

We investigate the role of leaky guided waves in transcranial ultrasound transmission in temporal and parietal bones at large incidence angles. Our numerical and experimental results show that the dispersion characteristics of the fundamental leaky guided wave mode with longitudinal polarization can be leveraged to estimate the critical angle above which efficient shear mode conversion takes place, and below which major transmission drops can be expected. Simulations that employ a numerical propagator matrix and a Semi-Analytical approach establish the transcranial dispersion characteristics and transmission coefficients at different incident angles. Experimental transmission tests conducted at 500 kHz and radiation tests performed in the 200-800 kHz range confirm the numerical findings in terms of transmitted peak pressure and frequency-radiation angle spectra, based on which the connection between critical angles, dispersion and transmission is demonstrated. Our results support the identification of transcranial ultrasound strategies that leverage shear mode conversion, which is less sensitive to phase aberrations compared to normal incidence ultrasound. These findings can also enable higher transmission rates in cranial bones with low porosity by leveraging dispersion information extracted through signal processing, without requiring measurement of geometric and mechanical properties of the cranial bone.

The Usefulness of Serum Brain Damage Biomarkers in Detection and Evaluation of Hypoxic Ischemic Encephalopathy in Calves with Perinatal Asphyxia.

The purpose of the present study was to determine hypoxic brain damage in calves with perinatal asphyxia using brain-specific damage biomarkers. Ten healthy and 25 calves with perinatal asphyxia were enrolled in the study. Clinical examination, neurological status score, and laboratory analysis were performed at admission, 24, 48, and 72 h. Serum concentrations of ubiquitin carboxy-terminal hydrolysis 1 (UCHL1), calcium-binding protein B (S100B), adrenomodullin (ADM), activitin A (ACTA), neuron-specific enolase (NSE), glial fibrillary acidic protein (GFAP) and creatine kinase-brain (CK-B) were measured. Histopathological and immunohistochemical examinations of the brain tissue were performed in 13 nonsurvivor calves. The neurological status score of the calves with asphyxia was significantly (p < 0.05) lower. Mix metabolic-respiratory acidosis and hypoxemia were detected in calves with asphyxia. Serum UCHL1 and S100B were significantly (p < 0.05) increased, and NSE, ACTA, ADM, and CK-B were decreased (p < 0.05) in calves with asphyxia. Histopathological and immunohistochemical examinations confirmed the development of mild to severe hypoxic-ischemic encephalopathy. In conclusion, asphyxia and hypoxemia caused hypoxic-ischemic encephalopathy in perinatal calves. UCHL1 and S100B concentrations were found to be useful markers for the determination of hypoxic-ischemic encephalopathy in calves with perinatal asphyxia. Neurological status scores and some blood gas parameters were helpful in mortality prediction.

Flapping dynamics of an inverted flag behind a cylinder.

The inverted flag configuration is inspired by biological structures (e.g. leaves on a tree branch), showing rich dynamics associated with instabilities at lower flow speeds than the regular flag configuration. In the biological counterpart, the arrangement of leaves and twigs on foliage creates a complex interacting environment that promotes certain dynamic fluttering modes. While enabling a large amplitude response for reduced flow speeds is advantageous in emerging fields such as energy harvesting, still, little is known about the consequence of such interactions. In this work, we numerically study the canonical bio-inspired problem of the flow-structural interaction of a 2D inverted flag behind a cylindrical bluff body, mimicking a leaf behind a tree branch, to investigate its distinct fluttering regimes. The separation distance between the cylinder and flag is gradually modified to determine the effective distance beyond which small-amplitude or large-amplitude flapping occurs for different flow velocities. It is shown that the flag exhibits a periodic large amplitude-low frequency response mode when the cylinder is placed at a sufficiently large distance in front of the flag. At smaller distances, when the flag is within the immediate wake of the cylinder, the flag undergoes a high frequency-small amplitude response. Finally, the flag's piezoelectric power harvesting capability is investigated numerically and experimentally for varying geometrical and electrical parameters associated with these two conditions. Two separate optimal response modes with the highest energy output have also been identified.

Photo-responsive hydrogel-based re-programmable metamaterials.

This paper explores a novel programmable metamaterial using stimuli-responsive hydrogels with a demonstration of bandgap formation and tuning. Specifically, a photo-responsive hydrogel beam that can achieve re-programmable periodicity in geometric and material properties through patterned light irradiation is designed. Hydrogels consist of polymeric networks and water molecules. Many unique properties of hydrogels, including bio-compatibility, stimuli-responsiveness, and low dissipation make them ideal for enabling re-programmable metamaterials for manipulating structural dynamic response and wave propagation characteristics. Bandgap generation and tunability in photo-responsive hydrogel-based metamaterial (in the form of a diatomic phononic chain) as well as the effects of system parameters such as light exposure pattern and photo-sensitive group concentration on the bandgap width and center frequency are systematically studied. In agreement with finite-element model simulations, it is observed that an increase in light exposure region size reduces both the bandgap width and center frequency, while an increase in the concentration of photo-sensitive group increases bandgap width, attenuation and reduces its center frequency. This work unveils the potential of stimuli-response hydrogels as a new class of low-loss soft metamaterials, unlike most other soft materials that are too lossy to sustain and exploit wave phenomena.

Biomarkers in premature calves with and without respiratory distress syndrome.

BACKGROUND: Approaches to the evaluation of pulmonary arterial hypertension (PAH) in premature calves by using lung-specific epithelial and endothelial biomarkers are needed. OBJECTIVE: To investigate the evaluation of PAH in premature calves with and without respiratory distress syndrome (RDS) by using lung-specific epithelial and endothelial biomarkers and determine the prognostic value of these markers in premature calves. ANIMALS: Fifty premature calves with RDS, 20 non-RDS premature calves, and 10 healthy term calves. METHODS: Hypoxia, hypercapnia, and tachypnea were considered criteria for RDS. Arterial blood gases (PaO2 , PaCO2 , oxygen saturation [SO2 ], base excess [BE], and serum lactate concentration) were measured to assess hypoxia. Serum concentrations of lung-specific growth differentiation factor-15 (GDF-15), asymmetric dimethylarginine (ADMA), endothelin-1 (ET-1), vascular endothelial growth factor (VEGF), and surfactant protein D (SP-D) were measured to assess PAH. RESULTS: Arterial blood pH, PaO2 , SO2 , and BE of premature calves with RDS were significantly lower and PaCO2 and lactate concentrations higher compared to non-RDS premature and healthy calves. The ADMA and SP-D concentrations of premature calves with RDS were lower and serum ET-1 concentrations higher than those of non-RDS premature and healthy calves. No statistical differences for GDF-15 and VEGF were found among groups. CONCLUSIONS AND CLINICAL IMPORTANCE: Significant increases in serum ET-1 concentrations and decreases in ADMA and SP-D concentrations highlight the utility of these markers in the diagnosis of PAH in premature calves with RDS. Also, we found that ET-1 was a reliable diagnostic and prognostic biomarker for PAH and predicting mortality in premature calves.

Trout-like multifunctional piezoelectric robotic fish and energy harvester.

This work presents our experimental studies on a trout-inspired multifunctional robotic fish as an underwater swimmer and energy harvester. Fiber-based flexible piezoelectric composites with interdigitated electrodes, specifically macro-fiber composite (MFC) structures, strike a balance between the deformation and actuation force capabilities to generate hydrodynamic propulsion without requiring additional mechanisms for motion amplification. A pair of MFC laminates bracketing a passive fin functions like artificial muscle when driven out of phase to expand and contract on each side to create bending. The trout-like robotic fish design explored in this work was tested for both unconstrained swimming in a quiescent water tank and under imposed flow in a water tunnel to estimate the maximum swimming speed, which exceeded 0.25 m s-1, i.e., 0.8 body lengths per second. Hydrodynamic thrust characterization was also performed in a quiescent water setting, revealing that the fin can easily produce tens of mN of thrust, similar to its biological counterpart for comparable swimming speeds. Overall, the prototype presented here generates thrust levels higher than other smart material-based concepts (such as soft polymeric material-based actuators which provide large deformation but low force), while offering simple design, geometric scalability, and silent operation unlike motor-based robotic fish (which often use bulky actuators and complex mechanisms). Additionally, energy harvesting experiments were performed to convert flow-induced vibrations in the wake of a cylindrical bluff body (for different diameters) in a water tunnel. The shed vortex frequency range for a set of bluff body diameters covered the first vibration mode of the tail, yielding an average electrical power of 120 µW at resonance for a flow speed around 0.3 m s-1and a bluff body diameter of 28.6 mm. Such low-power electricity can find applications to power small sensors of the robotic fish in scenarios such as ecological monitoring, among others.

Experimental Observation of Temporal Pumping in Electromechanical Waveguides.

We experimentally demonstrate temporal pumping of elastic waves in an electromechanical waveguide. Temporal pumping exploits a virtual dimension mapped to time, enabling the generation and control of edge states, typical of two-dimensional systems, in a one-dimensional waveguide. We show experimentally that the temporal modulation of the stiffness drives the transfer of edge states from one boundary of the waveguide to the other. The considered implementation, that consists of an elastic waveguide coupled with tunable electrical impedances, allows the pumping to occur in a controllable manner. The framework presented herein opens new avenues for the manipulation and transport of information through elastic waves, with potential technological applications for digital delay lines and digitally controlled waveguides. This Letter also explores higher-dimensional topological physics using virtual dimensions mapped to time in electromechanical systems.

Experimental identification of high order Lamb waves and estimation of the mechanical properties of a dry human skull.

We experimentally investigate and characterize high order Lamb wave modes in a dry human skull. Specifically, we show that the diploë supports distinct wave modes in the sub-1.0 MHz frequency regime, and we employ these modes for the estimation of equivalent mechanical properties of cortical and trabecular bones. These modes are efficiently generated in a parietal region by direct contact excitation with a wedge beam transducer, and are recorded via infrared laser vibrometry. Frequency/wavenumber data are estimated using a matrix pencil method applied to wavefield measurements recorded on the outer cortical surface. The semi-analytical finite element model of an equivalent three-layered plate provides the platform for the identification of wave modes based on their through-the-thickness profiles, and supports the estimation of equivalent mechanical properties in conjunction with an optimization algorithm developed for this purpose. The results presented herein illustrate how high order Lamb waves can be used to gain understanding of the wave properties of a human skull and to estimate the orthotropic and equivalent isotropic mechanical properties of cortical and trabecular bones.

Radiation Characteristics of Cranial Leaky Lamb Waves.

We numerically and experimentally investigate the dispersion properties of leaky Lamb waves in the cranial bone. Cranial Lamb waves leak energy from the skull into the brain when propagating at speeds higher than the speed of sound in the surrounding fluid. The understanding of their radiation mechanism is significantly complicated by the geometric and mechanical characteristics of the cortical tables and the trabecular bone (diploë). Toward such understanding, we here analyze the sub-1.0 MHz radiation angle dispersion spectrum of porous bone phantoms and parietal bone geometries obtained from µ CT scans. Our numerical results show that, when diploic pores are physically modeled, leakage angles computed from time transient finite-element analyses correspond to those predicted by an equivalent three-layered fluid-loaded waveguide model. For the bone geometries analyzed, two main leaky branches are observed in the near-field dispersion spectrum: a fast wave radiated at small angles, which is related to the fastest fundamental Lamb mode supported by the cranial bone, and a slower wave radiated at larger angles. This observation is also confirmed by experimental tests carried out on an immersed parietal bone.

Vibration Stimulation as a Non-Invasive Approach to Monitor the Severity of Meniscus Tears.

Musculoskeletal disorders and injuries are one of the most prevalent medical conditions across age groups. Due to a high load-bearing function, the knee is particularly susceptible to injuries such as meniscus tears. Imaging techniques are commonly used to assess meniscus injuries, though this approach suffers from limitations including high cost, need for skilled personnel, and confinement to laboratory or clinical settings. Vibration-based structural monitoring methods in the form of acoustic emission analysis and vibration stimulation have the potential to address the limits associated with current diagnostic technologies. In this study, an active vibration measurement technique is employed to investigate the presence and severity of meniscus tear in cadaver limbs. In a highly controlled ex vivo experimental design, a series of cadaver knees (n =6) were evaluated under an external vibration, and the frequency response of the joint was analyzed to differentiate the intact and affected samples. Four stages of knee integrity were considered: baseline, sham surgery, meniscus tear, and meniscectomy. Analyzing the frequency response of injured legs showed significant changes compared to the baseline and sham stages at selected frequency bandwidths. Furthermore, a qualitative analytical model of the knee was developed based on the Euler-Bernoulli beam theory representing the meniscus tear as a change in the local stiffness of the system. Similar trends in frequency response modulation were observed in the experimental results and analytical model. These findings serve as a foundation for further development of wearable devices for detection and grading of meniscus tear and for improving our understanding of the physiological effects of injuries on the vibration characteristics of the knee. Such systems can also aid in quantifying rehabilitation progress following reconstructive surgery and / or during physical therapy.

Experimental and Computational Investigation of Guided Waves in a Human Skull.

We investigate guided (Lamb) waves in a human cadaver skull through experiments and computational simulations. Ultrasonic wedge transducers and scanning laser Doppler vibrometry are used respectively to excite and measure Lamb waves propagating in the cranial bone of a degassed skull. Measurements are performed over a section of the parietal bone and temporal bone spanning the squamous suture. The experimental data are analyzed for the identification of wave modes and the characterization of dispersion properties. In the parietal bone, for instance, the A0 wave mode is excited between 200 and 600 kHz, and higher-order Lamb waves are excited from 1 to 1.8 MHz. From the experimental dispersion curves and average thickness extracted from the skull computed tomography scan, we estimate average isotropic material properties that capture the essential dispersion characteristics using a semi-analytical finite-element model. We also explore the leaky and non-leaky wave behavior of the degassed skull with water loading in the cranial cavity. Successful excitation of leaky Lamb waves is confirmed (for higher-order wave modes with phase velocity faster than the speed of sound in water) from 500 kHz to 1.5 MHz, which may find applications in imaging and therapeutics at the brain periphery or skull-brain interface (e.g., for metastases). The non-leaky A0 Lamb wave mode propagates between 200 and 600 kHz, with or without fluid loading, for potential use in skull-related diagnostics and imaging (e.g., for sutures).

Vibration Sensing Systems Based on Poly(Vinylidene Fluoride) and Microwave-Assisted Synthesized ZnO Star-Like Particles with Controllable Structural and Physical Properties.

This study deals with the effect of zinc oxide (ZnO) star-like filler addition to the poly(vinylidene fluoride) (PVDF) matrix, and its effect on the structural and physical properties and consequences to the vibration sensing performance. Microwave-assisted synthesis in open vessel setup was optimized for the preparation of the star-like shape of ZnO crystalline particles. The crystalline and star-like structure was confirmed by X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDX). Furthermore, the PVDF-based composites were prepared using a spin-coating technique from solution. An investigation of the transformation of the α crystalline phase to the ß crystalline phase of the neat PVDF matrix and with various filler concentrations was performed using Fourier-Transform infrared (FTIR) spectroscopy, which shows an enhanced ß-phase from 44.1% to 66.4% for neat PVDF and PVDF with 10 wt.% of particles, respectively. Differential scanning calorimetry (DSC) measurements and investigation showed enhanced crystallinity and melting enthalpy of the composite systems in comparison to neat PVDF, since ZnO star-like particles act as nucleating agents. The impact of the filler content on the physical properties, such as thermal and dynamic mechanical properties, which are critical for the intended applications, were investigated as well, and showed that fabricated composites exhibit enhanced thermal stability. Because of its dynamic mechanical properties, the composites can still be utilized as flexible sensors. Finally, the vibration sensing capability was systematically investigated, and it was shown that the addition of ZnO star-like filler enhanced the value of the thickness mode d33 piezoelectric constant from 16.3 pC/N to 29.2 pC/N for neat PVDF and PVDF with 10 wt.% of ZnO star-like particles.

Vibration Characterization of the Human Knee Joint in Audible Frequencies.

Injuries and disorders affecting the knee joint are very common in athletes and older individuals. Passive and active vibration methods, such as acoustic emissions and modal analysis, are extensively used in both industry and the medical field to diagnose structural faults and disorders. To maximize the diagnostic potential of such vibration methods for knee injuries and disorders, a better understanding of the vibroacoustic characteristics of the knee must be developed. In this study, the linearity and vibration transmissibility of the human knee were investigated based on measurements collected on healthy subjects. Different subjects exhibit a substantially different transmissibility behavior due to variances in subject-specific knee structures. Moreover, the vibration behaviors of various subjects' knees at different leg positions were compared. Variation in sagittal-plane knee angle alters the transmissibility of the joint, while the overall shape of the transmissibility diagrams remains similar. The results demonstrate that an adjusted stimulation signal at frequencies higher than 3 kHz has the potential to be employed in diagnostic applications that are related to knee joint health. This work can pave the way for future studies aimed at employing acoustic emission and modal analysis approaches for knee health monitoring outside of clinical settings, such as for field-deployable diagnostics.

Aspect Ratio-Dependent Dynamics of Piezoelectric Transducers in Wireless Acoustic Power Transfer.

Acoustic power transfer (APT) for wireless electronic components has received growing attention as a viable approach to deliver power to remotely located small electronic devices. The design of an efficient APT system requires accurate models to describe its individual components as well as the interaction between them. Most of the analytical models available to represent the bulk piezoelectric transducers used in APT are limited to either thin rod or thin plate transducers. However, transducers with moderate aspect ratios are often used, especially at the receiver end. In this work, in addition to reviewing standard theories, models based on the Rayleigh and Bishop rod theories are developed to analyze transducers [transmitter (TX) or receiver (RX)] with various aspect ratios. Results from these models are compared with experimental data and finite-element analysis to determine the range of aspect ratios in which they are valid. In addition, fluid loading effects on the predictions of all models are investigated, and the generated pressure fields by the transducers with different aspect ratios are compared. The resulting models are used to analyze the effect of aspect ratio on the performance of the transducer when operated as a TX or an RX in an APT setting.

Use of intestine-related biomarkers for detecting intestinal epithelial damage in neonatal calves with diarrhea.

OBJECTIVE: To evaluate the usefulness of intestinal biomarkers in determining the presence of intestinal epithelial damage in neonatal calves with diarrhea caused by 4 etiologic agents. ANIMALS: 40 neonatal calves that were healthy (n = 10) or had diarrhea (30). PROCEDURES: The study was a cross-sectional study. Results of hematologic analyses and serum concentrations of intestinal fatty acid-binding protein (I-FABP), liver fatty acid-binding protein (L-FABP), trefoil factor 3 (TFF-3), Claudin-3 (CLDN-3), γ-enteric smooth muscle actin (ACTG2), intestinal alkaline phosphatase (IAP), interleukin-8 (IL-8), platelet-activating factor (PAF), and leptin (LP) were compared among calves grouped according to whether they were healthy (control group; G-1) or had diarrhea caused by K99 Escherichia coli (G-2; n = 10), bovine rota- or coronavirus (G-3; 5 each), or Cryptosporidium spp (G-4; 10). RESULTS: Across the 3 time points at which blood samples were obtained and evaluated, the groups of calves with diarrhea generally had markedly higher mean serum concentrations of L-FABP, TFF-3, IAP, IL-8, and LP, compared with the control group. In addition, G-2 also consistently had markedly higher mean serum concentrations of I-FAB and ACTG2 and lower mean serum concentrations of CLDN-3, compared with the control group. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that degree of intestinal epithelial damage differed among calves grouped by the etiologic agent of diarrhea and that such damage might have been more severe in calves with diarrhea caused by K99 E coli. Additionally, our results indicated that serum concentrations of I-FABP, L-FABP, TFF-3, IAP, IL-8, ACTG2, LP, and CLDN-3 were useful biomarkers of intestinal epithelial damage in calves of the present study.

Heart-type fatty acid-binding protein (H-FABP), pentraxin-3 (PTX-3) and thrombomodulin in bovine traumatic pericarditis.

The aim of this study was to evaluate the biomarkers of cardiac damage such as heart-type fatty acid-binding protein (H-FABP), pentraxin-3 (PTX-3), and thrombomodulin (TM) for the detection and prognosis of bovine traumatic pericarditis (TP). Spontaneous TP was diagnosed on the basis of history, clinical signs, complete blood count, glutaraldehyde test, ultrasonography, and pericardiocentesis findings. H-FABP, PTX-3 and TM levels in serum were compared between 25 Holstein cows diagnosed with spontaneous TP and 10 healthy control cows using bovine-specific ELISA kits. Serum H-FABP in cattle with TP was significantly (P < 0.05) higher than in the control group and positively correlated with cardiac troponin-I (cTnI), creatine kinase myocardial band (CK-MB), PTX-3 and TM (r = 0.683, 0.342, 0.448 and 0.424, respectively; P < 0.05). The serum levels of PTX-3 (P < 0.05) and TM (P < 0.05) in cattle with TP were significantly higher than in the control group. Cardiac damage biomarkers H-FABP, PTX-3 and TM may be useful in the diagnosis of bovine TP.